You have to. For example if you were investigating the affect of temperature on an enzyme you would have to keep many other variables constant and controlled such as pH,volume of the reaction,amount of enzyme, amount of substrate, time of reaction, etc....Why can you have more than one controlled variable in an experiment?
Edit:
actually you can have more than one controlled variable also called a constant variable... most experiments actually DO have these.
http://www.sciencebuddies.org/science-fa鈥?/a>
http://en.wikipedia.org/wiki/Experimenta鈥?/a>Why can you have more than one controlled variable in an experiment?
A controlled Variable has a constant, thus it is controlled.
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