Can you tell me the control,independent variable, and dependent variable for this experiment?

In this lab you will observe the activity of hydrogen peroxidase from potatoes and determine the speed


(rate) of reaction catalyzed by this enzyme.


Materials (per group)


10 50-mL plastic cups Sharpie Marker potato solution


3 50- or 100-mL Graduated cylinders labeling tape distilled water (15掳C)


1% hydrogen peroxide (H2O2) forceps 10 filter-paper disks


Procedure


1. Obtain five 50-mL small plastic cups and label them as follows: #1, 100% enzyme; #2, 75%


enzyme; #3, 50% enzyme; #4, 25% enzyme, #5, 0% enzyme. You can write directly on the cups


with the Sharpies.


2. Obtain three graduated cylinders and label them with labeling tape and a marker as follows: #1,


enzyme; #2, distilled H2O; #3, 1% H2O2. Set aside graduated cylinder #3 for use later. Only use


the graduated cylinders for the appropriate liquid鈥攁void cross contamination at all costs!


Y


2H2O2 Hydrogen Peroxidase 2H2O + O2


Substrate Enzyme Product(s)


3. Using the labeled graduated cylinders for the appropriate solutions, obtain potato solution and


distilled water. Prepare five solutions containing different concentrations of enzyme in the five


numbered beakers or cups according to the following mixtures:


Beaker # Enzyme (potato solution) Distilled H2O (15掳C)


1 (100%) 40 mL 0 mL


2 (75%) 30 mL 10 mL


3 (50%) 20 mL 20 mL


4 (25%) 10 mL 30 mL


5 (0%) 0 mL 40 mL


4. Obtain five more 50-mL cups and label them A, B, C, D, and E. Using the graduated cylinder


labeled 1% H2O2, add 40 mL of hydrogen peroxide (the substrate) to each beaker. The reactions


are now ready to run.


5. Using the forceps, obtain ten filter-paper disks. Set them on a dry paper towel.


6. Using the forceps, take one filter-paper disk and immerse it in beaker #1. Allow the disk to


absorb the enzyme solution for 5 seconds. Remove the disk and drain it for 10 seconds on a


paper towel. Drop the disk into beaker A. Observe the disk鈥檚 movement, and note the time


when the disk reaches the surface. Record the time in the Data Table. Convert the time from


seconds into minutes. Also record this in the Data Table.


7. Repeat step 6 for a second trial. Record your results in the Data Table.


8. Repeat the procedure in step 6 using beaker #2 and beaker B. Also run two trials.


9. Repeat steps 6 and 7 using beaker #3 and beaker C. Run two trials.


10. Repeat steps 6 and 7 using beaker #4 and beaker D. Run two trials.


11. Repeat steps 6 and 7 using beaker #5 and beaker E. Run two trials.


12. Calculate the reaction rate, or speed of the reactions. Divide the concentration of the enzyme


(remember to convert from percentage to decimal) by the time it took the disk to reach the


surface.Can you tell me the control,independent variable, and dependent variable for this experiment?
thedependent variable is the rate of reaction becase the it is affected by the different enzymes (the independent variable) i think so anyway didn't read most if the stuff you put up thereCan you tell me the control,independent variable, and dependent variable for this experiment?
the control is the substance that you did not add or do anything to